Treatment of adjuvant arthritis with granulocyte-colony stimulating factor and peptide derived from heat shock protein 65

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• 作者：Brendolan ; Andrea ; Higuchi ; Masanori ; Sibley ; Richard ; Strober ; Samuel
• 关键词：Adjuvant arthritis ; Granulocyte-colony stimulating factor ; Heat shock protein ; Immunoregulation
• 刊名：Cellular Immunology
• 出版年：2003
• 出版时间：January, 2003
• 年：2003
• 卷：221
• 期：1
• 页码：6-14
• 全文大小：424 K

文摘

Adjuvant arthritis in Lewis rats is induced by the subcutaneous injection of Mycobacterium tuberculosis in mineral oil, and the predominant T cell immune reactivity is against the heat shock protein 65 derived peptide 176–190. We treated Lewis rats with human recombinant G-CSF followed by (i.v) administration of peptide 176–190 after induction of adjuvant arthritis (AA), and observed decreased disease severity, joint destruction, new bone formation and joint ankylosis. Treatment with G-CSF alone was also effective, but to a lesser extent. In addition, we found that splenocytes from rats treated with G-CSF had reduced antigen presenting capacity compared with splenocytes from vehicle treated rats. Primed lymph node cells from G-CSF plus peptide treated rats showed a marked reduction in proliferation and secretion of IFN-γ after stimulation with the heat shock protein peptide in vitro as compared to controls.