An enhanced miniaturized assay for antimicrobial prospecting
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- 作者：Shannon M. Smith ; Andrew J. Beattie ; Michael R. Gillings ; Marita P. Holley ; Adam J. Stow ; Christine L. Turnbull ; Peter D. Wilson ; David A. Briscoe
- 关键词：Antimicrobials ; Assay ; Bioprospecting ; Microtitre
- 刊名：Journal of Microbiological Methods
- 出版年：2008
- 出版时间：January 2008
- 年：2008
- 卷：72
- 期：1
- 页码：103-106
- 全文大小：198 K
文摘
Bioprospecting for novel antimicrobials increasingly relies on extremely small samples unsuitable for conventional bulk extraction and assay. We developed a microtitre plate assay for minimal amounts of test materials which is rapid, extremely sensitive, allows time-course analysis and reduces false negatives. Developed for the analyses of antimicrobial sensitivity and resistance, the technique is appropriate for assays where source materials are scarce.