- 作者：Paula Brito e Cabral ; Jos¨¦ Ev ; ro Cunha J¨²nior ; Alex ; re Casimiro de Macedo ; Alex ; re Rodrigues Alves ; Thially Braga Gon?alves ; Tereza Cristina Brito e Cabral ; Ana Paula Soares Gondim ; Maria Isabel Moraes Pinto ; Karen Tubono Oseki ; Lilia Maria Carneiro Camara ; Silvia Helena Barem Rabenhorst ; Aparecida Tiemi Nagao-Dias
- 关键词：Mycobacterium leprae DNA ; Serum and salivary anti-PGL1 ; Leprosy household contacts
- 刊名：International Journal of Infectious Diseases
- 出版年：2013
- 出版时间：November, 2013
- 年：2013
- 卷：17
- 期：11
- 页码：e1005-e1010
- 全文大小：486 K
Summary
Objectives
Leprosy household contacts represent a group at high risk of developing the disease. The aim of this study was to detect Mycobacterium leprae subclinical infection in this group through serological and molecular parameters.Methods
Serum anti-PGL1 IgG/IgM and salivary anti-PGL1 IgA/IgM was investigated using an ELISA, and nasal carriage of M. leprae DNA was detected by PCR, in leprosy household contacts of paucibacillary (PB) and multibacillary (MB) household leprosy patients (n = 135), their index cases (n = 30), and in persons living in a low endemic city (n = 17).
Results
Salivary anti-PGL1 IgA and IgM and serum anti-PGL1 IgG showed good correlation comparing contacts and index cases (p < 0.01, p < 0.005, and p < 0.0001, respectively). This was not observed for serum anti-PGL1 IgM (p > 0.05). A high frequency of anti-PGL1 IgM positivity was found in IgG-negative samples (p < 0.0001). For IgG-positive samples, IgM antibodies were also positive in most of the samples. None of the 17 volunteers living in a low endemic city presented seropositivity for IgG; however, two of them showed positivity for anti-PGL1 IgM. M. leprae DNA was found in the nasal swabs of nine out of the 85 MB household leprosy contacts (10.6 % ) and in three out of the 50 PB household leprosy contacts (6.0 % ).
Conclusion
We strongly suggest that serum IgG/IgM and salivary anti-PGL1 IgA/IgM measurements are used to follow leprosy household contacts.