Addition of 3-nitrooxypropanol (NOP) reduced methane emissions by up to 86.2%.
Total volatile fatty acid (VFA) concentration was not affected by NOP.
The molar proportion of acetate was reduced by up to 8.7% with NOP.
Metabolic hydrogen was mainly re-directed to VFA when NOP was present.
Methanogens were reduced by NOP in the solid phase but not in the liquid phase.