Liquid chromatography-tandem mass spectrometric determination of propofol in rat serum and hair at attogram level after derivatization with 3-bromomethyl-propyphenazone
刊名:Journal of Pharmaceutical and Biomedical Analysis
出版年:2017
出版时间:5 February 2017
年:2017
卷:134
期:Complete
页码:195-202
全文大小:1250 K
卷排序:134
文摘
Derivatization of propofol with 3-bromomethyl-propyphenazone followed by LVC/MS analysis. The derivatized propofol was detectable at attogram level in hair and serum. The LLOQ was 0.01 pg/mL serum (equivalent to 50 ag/μL in injectable solution). The ionization suppression was minimized by deactivating the excess reagent with methanol. The concentration patterns of propofol in rat serum and hair were unmatched.