Identification and characterisation of new inhibitors for the human hematopoietic prostaglandin D2 synthase
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- 作者：Jane E. Weber ; Aaron J. Oakley ; Angelika N. Christ ; Alan G. Clark ; John D. Hayes ; Rhonda Hall ; David A. Hume ; Philip G. Board ; Mark L. Smythe ; Jack U. Flanagan
- 关键词：Prostaglandin synthase ; Docking ; Structure ; Inhibition ; Glutathione transferase
- 刊名：European Journal of Medicinal Chemistry
- 出版年：2010
- 出版时间：February 2010
- 年：2010
- 卷：45
- 期：2
- 页码：447-454
- 全文大小：861 K
文摘
Prostaglandin D2 synthesised by the hematopoietic prostaglandin D2 synthase has a pro-inflammatory effect in allergic asthma, regulating many hallmark characteristics of the disease. Here we describe identification of hematopoietic prostaglandin D2 synthase inhibitors including cibacron blue, bromosulfophthalein and ethacrynic acid. Expansion around the drug-like ethacrynic acid identified a novel inhibitor, nocodazole, and a fragment representing its aromatic core. Nocodazole binding was further characterised by docking calculations in combination with conformational strain analysis. The benzyl thiophene core was predicted to be buried in the active site, binding in the putative prostaglandin binding site, and a likely hydrogen bond donor site identified. X-ray crystallographic studies supported the predicted binding mode.