Improved biovar test for Ralstonia solanacearum

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• 作者：Qi Huang^a ; ^{qi.huang@ars.usda.gov} ; Xuerui Yan^a ; ¹ ; Jaw-Fen Wang^b
• 关键词：r3b2 ; race 3 biovar 2
• 刊名：Journal of Microbiological Methods
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：88
• 期：2
• 页码：271-274
• 全文大小：620 K

文摘

Race 3, biovar 2 strains of Ralstonia solanacearum are quarantined pathogens in Europe and Canada and Select Agent pathogens in the United States. The biovar classification of R. solanacearum strains is based on their biochemical abilities to utilize a carbohydrate panel. The standard biovar test uses bromothymol blue as a pH indicator in 15 ml culture tubes containing 3 to 5 ml of test media, and takes weeks to complete at 24 or 28 ¡ãC. We improved the biovar test by using phenol red as a pH indicator that changes color at a higher pH when a carbohydrate is utilized. We also conducted the test at 32 ¡ãC in 0.2 ml of 8-tube strips that reduced the medium needed by at least 20 fold. Using the improved test, biovars of R. solanacearum strains can be determined in 4 days when a panel of seven carbohydrates is used including glucose, trehalose, mannitol, sorbitol, dulcitol, maltose and cellobiose. To differentiate biovars 1, 2, 3 and 4, the test can be further simplified and completed in 3 days using a panel of four carbohydrates containing glucose, trehalose, maltose and dulcitol, significantly saving money, space and time.