NADH dehydrogenase of Trypanosoma brucei is important for efficient acetate production in bloodstream forms

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• 作者：Sachin V. Surve^a ; ¹ ; Bryan C. Jensen^a ; Meredith Heestand^a ; ² ; Muriel Mazet^b ; ³ ; Terry K. Smith^c ; Fré ; dé ; ric Bringaud^b ; ³ ; Marilyn Parsons^a ; ^d ; ⁴ ; ^{marilyn.parsons@cidresearch.org} ; Achim Schnaufer^e ; ⁴ ; ^{achim.schnaufer@ed.ac.uk}
• 关键词：Trypanosoma brucei ; Mitochondrion ; NDH2 ; Respiratory complex I ; NADH ; ubiquinone oxidoreductase ; Acetate
• 刊名：Molecular and Biochemical Parasitology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：211
• 期：Complete
• 页码：57-61
• 全文大小：1220 K
• 卷排序：211

文摘

Various genetic mutants of NDH2 were created in bloodstream form Trypanosoma brucei. NDH2 null mutants showed a substantial reduction in growth. NDH2 ablation in a complex I deficient background led to severe growth restriction. Upon prolonged culture, parasites partially compensated for NDH2 deficiency. Loss of NDH2 led to reduced acetate, potentially contributing to the growth defect.