Quantification of selected furocoumarins by high-performance liquid chromatography and UV-detection: Capabilities and limits
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- 作者：Angus P. Macmaster ; Neil Owen ; Sylvain Brussaux ; Hugues Brevard ; Richard Hiserodt ; Hans Leijs ; Nikola Bast ; Berthold Weber ; Gerd Loesing ; Alan Sherlock ; Christine Schippa ; Matthias Vey ; Eric Fr¨¦rot ; Emeline Tissot ; Alain Chaintreau
- 关键词：Furocoumarin ; Quantification ; HPLC&ndash ; UV ; Validation ; Accuracy ; Limit of quantification
- 刊名：Journal of Chromatography A
- 出版年：2012
- 出版时间：28 September, 2012
- 年：2012
- 卷：1257
- 期：Complete
- 页码：34-40
- 全文大小：742 K
文摘
The performance of HPLC-UV as a means of quantifying selected furocoumarins in essential oils has been evaluated, based on a ring test validation approach. Accuracy profiles were generated, to determine bias and statistical confidence associated with determination at different concentrations, along with lower limits of quantification (LOQ). From these findings, it can be concluded that the method described may only be used in simple cases (essential oils), to measure individual furocoumarin compounds at concentrations greater than 10 mg/l; the non compound-specific nature of detection by absorption in the UV range is unable to overcome the effect of interferences arising from chromatographic coelutions, such as those encountered in the analysis of complex commercial fragrance mixtures. The use of an algorithmically calculated ¡®spectral similarity¡¯ function, with reference to authentic standards, may be used to improve reliability in assignment and quantification.