Protein structure determination by electron cryo-microscopy

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• 作者：Slavica Jonic ; Catherine Vé ; nien-Bryan
• 刊名：Current Opinion in Pharmacology
• 出版年：2009
• 出版时间：October 2009
• 年：2009
• 卷：9
• 期：5
• 页码：636-642
• 全文大小：1272 K

文摘

Transmission electron cryo-microscopy (cryoEM) is a versatile tool in the structural analysis of proteins and biological macromolecular assemblies. In this review, we present a brief survey of the methods used in cryoEM, and their current developments. These latest advances provide exciting opportunities for the three-dimensional structural determination of macromolecular complexes that are either too large or too heterogeneous to be investigated by conventional X-ray crystallography or nuclear magnetic resonance (NMR). The endeavour of understanding the function of protein or macromolecular complex is often helped by combining data from electron microscopy and X-ray crystallography. We will thus provide a brief overview of the computational techniques involved in combining data from different techniques for the interpretation of the EM structure.