Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology

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• 作者：Madalena V. Cruz^a ; Alexandre Paiva^a ; Pedro Lisboa^a ; Filomena Freitas^a ; Ví ; tor D. Alves^b ; Pedro Simõ ; es^a ; Susana Barreiros^a ; Maria A.M. Reis^a ; ^{amr@fct.unl.pt" class="auth_mail}
• 关键词：Polyhydroxyalkanoates (PHAs) ; Cupriavidus necator ; Spent coffee grounds (SCG) ; Triglycerides ; Supercritical fluid extraction (SFE)
• 刊名：Bioresource Technology
• 出版年：April, 2014
• 年：2014
• 卷：157
• 期：Complete
• 页码：360-363
• 全文大小：468 K

文摘

Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO₂) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35 kg kg^鈭? CO₂/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2 L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7 g L^鈭? with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7 g L^鈭? day^鈭? and 0.77 g g^鈭?, respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34 脳 10⁵ and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16 MPa and Young鈥檚 Modulus of 1.0 GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties.