A novel mammalian bubblegum-related acyl-CoA synthetase restricted to testes and possibly involved in spermatogenesis
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- 作者：Peter Fraisl ; Hiromitsu Tanaka ; Sonja Forss-Petter ; Hans Lassmann ; Yoshitake Nishimune and Johannes Berger
- 关键词：Acyl-CoA synthetase ; Bubblegum related ; Lipidosin related ; Testis ; Upstream ORF ; Very long-chain fatty acid
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2006
- 出版时间：1 July 2006
- 年：2006
- 卷：451
- 期：1
- 页码：23-33
- 全文大小：771 K
文摘
We have characterized a new, membrane-associated acyl-CoA synthetase (ACS), termed bubblegum-related protein (BGR), which upon functional analysis demonstrated ACS activity capable of activating long- and very long-chain fatty acids. By multiple tissue RNA array and Northern blot analyses, human BGR mRNA was exclusively detected in testes. Murine Bgr mRNA was specifically expressed in pubertal and adult testes and was further demonstrated to be enriched in germ cells and Sertoli cells while present at a lower level in Leydig cells both by in situ hybridization and cell type fractionation. The complex 5′-end of the BGR mRNA appears to underlie translational control leading to differential utilization of alternative translation start sites. Thus, the BGR gene expands the bubblegum ACS family with a testes-specific, developmentally regulated member that may play a role in spermatogenesis.