ASE extraction method for simultaneous carbon and nitrogen stable isotope analysis in soft tissues of aquatic organisms

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• 作者：Nathalie Bodin ; Hé ; lè ; ne Budzinski ; Karyn Le Mé ; nach ; Nathalie Tapie
• 关键词：δ ; ¹⁵N and δ ; ¹³C ; Accelerated solvent extraction ; Fat content ; Muscle ; Fish
• 刊名：Analytica Chimica Acta
• 出版年：2009
• 出版时间：8 June 2009
• 年：2009
• 卷：643
• 期：1-2
• 页码：54-60
• 全文大小：301 K

文摘

Since lipids are depleted in ¹³C relative to proteins and carbohydrates, variations in lipid composition among species and within individuals significantly influence δ¹³C and may result in misleading ecological interpretations. Whereas lipid extraction before IRMS analysis constitutes a way of stable isotope result lipid-normalisation, such a procedure was given up because of the un-controlled effects of the methods used (i.e., “Bligh & Dyer”, Soxhlet, etc.) on δ¹⁵N. The aim of this work was to develop a simple, rapid and efficient lipid extraction method allowing for simultaneous C and N stable isotope analysis in the biological soft tissues of aquatic organisms. The goal was to be free from the lipid influence on δ¹³C values without interfering with δ¹⁵N values. For that purpose, the modern automated pressurized liquid extraction technique ASE (accelerated solvent extraction) was selected. Eel muscles representative of a broad range of fat contents were extracted via ASE by using different semi-polar solvents (100 % dichloromethane and 80 % n-hexane/20 % acetone) and by operating at different temperature (ambient temperature and 100 °C) and pressure (750 and 1900 psi) conditions. The results were discussed in terms of lipid extraction efficiency as well as δ¹³C and δ¹⁵N variability.