Quantitative on-line preconcentration-liquid chromatography coupled with tandem mass spectrometry method for the determination of pharmaceutical compounds in water

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• 作者：Salima Idder ; Laurent Ley ; Patrick Mazellier ; H茅l猫ne Budzinski
• 关键词：On-line preconcentration ; Liquid chromatography&ndash ; mass spectrometry (LC&ndash ; MS/MS) ; Multi-residue analysis ; Pharmaceuticals ; Water analysis ; Environment
• 刊名：Analytica Chimica Acta
• 出版年：17 December, 2013
• 年：2013
• 卷：805
• 期：Complete
• 页码：107-115
• 全文大小：1036 K

文摘

One of the current environmental issues concerns the presence and fate of pharmaceuticals in water bodies as these compounds may represent a potential environmental problem. The characterization of pharmaceutical contamination requires powerful analytical method able to quantify these pollutants at very low concentration (few ng L^鈭?).

In this work, a multi-residue analytical methodology (on-line solid phase extraction-liquid chromatography-triple quadrupole mass spectrometry using positive and negative electrospray ionization) has been developed and validated for 40 multi-class pharmaceuticals and metabolites for tap and surface waters. This on-line SPE method was very convenient and efficient compared to classical off-line SPE method because of its shorter total run time including sample preparation and smaller sample volume (1 mL vs up to 1 L). The optimized method included several therapeutic classes as lipid regulators, antibiotics, beta-blockers, non-steroidal anti-inflammatories, antineoplastic, etc., with various physicochemical properties. Quantification has been achieved with the internal standards. The limits of detection are between 0.7 and 15 ng L^鈭? for drinking waters and 2-15 ng L^鈭? for surface waters. The inter-day precision values are below 20% for each studied level.

The improvement and strength of the analytical method has been verified along a monitoring of these 40 pharmaceuticals in Isle River, a French stream located in the South West of France. During this survey, 16 pharmaceutical compounds have been detected.