Characterization and expression analysis of serpins in the Chinese mitten crab Eriocheir sinensis
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- 作者：Qing Li1 ; Lihua Liu1 ; Yang Wang ; Jing Xie ; Lin He ; lhe@bio.ecnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Qun Wang ; qwang@bio.ecnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：SPIs ; serpin protease inhibitors ; AR ; acrosome reaction ; BCA ; bicinchoninic acid ; DAPI ; 4 ; 6-diamidino-2-phenylindole ; OCT ; optimal cutting temperature ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; TBST ; Tris buffered saline with Tween 20 ; PBS ; phosphate buffered saline ; BSA ; bovine serum albumin ; RCL ; reactive center loop ; PAP ; prophenoloxidase activating proteases ; SFT ; seminiferous tubule ; SG ; spermatogonium ; SC ; spermatocytes ; ST ; spermatid ; AG ; accessory gland
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：632-640
- 全文大小：2273 K
文摘
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The full-length cDNA of the Esserpin-2 and Esserpin-3 genes were cloned.
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Esserpin-2 and Esserpin-3 share common features with other serpin genes.
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Spatial and temporal distribution of Esserpin-3 expression was observed.
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20">Esserpin-3 expression increased during the development of the testis.
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Esserpin-3 expression reduced during the acrosome reaction.