Reevaluating the Sperm Nuclear Chromatin Decondensation Test by Sodium Thioglycolate of Stallions Spermatozoa
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- 作者：Ricardo Hartleya ; b ; Pablo Arosb ; Eduardo Bustos-Obregó ; nc ; Fernando Romerod ; Marco Alvarengae ; Alfredo Ramí ; rez-Revecob ; alfredoramirez@uach.cl" class="auth_mail" title="E-mail the corresponding author
- 关键词：Stallion ; Sperm nucleus ; Chromatin compaction ; Sodium thioglycolate
- 刊名：Journal of Equine Veterinary Science
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：36
- 期：Complete
- 页码：10-14
- 全文大小：733 K
文摘
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Was tested nuclear compaction protocol of Fornés and Bustos-Obregón
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We observed that only dead sperm cell react with thioglycolate
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The protocol was modified using different approach to make it reproducible
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Was developed a protocol to evaluate nuclear compaction state in equine sperm cell