Alterations in the cholinesterase and adenosine deaminase activities and inflammation biomarker levels in patients with multiple sclerosis

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• 作者：C.R.N. Polachini<sup>asup><sup> ; sup><sup>carlinha_polachini@hotmail.com" class="auth_mailsup> ; R.M. Spanevello<sup>bsup> ; E.A. Casali<sup>csup> ; D. Zanini<sup>asup> ; L.B. Pereira<sup>asup> ; C.C. Martins<sup>asup> ; J. Baldissareli<sup>asup> ; A.M. Cardoso<sup>asup> ; M.F. Duarte<sup>dsup> ; P. da Costa<sup>asup> ; A.L.C. Prado<sup>esup> ; M.R.C. Schetinger<sup>asup> ; V.M. Morsch<sup>asup><sup> ; sup><sup>veramorsch@gmail.com" class="auth_mailsup>
• 关键词：ACh ; acetylcholine ; AChE ; acetylcholinesterase ; ADA ; adenosine deaminase ; BChE ; butyrylcholinesterase ; BuSCh ; butyrylthiocholine iodide ; CRP ; C-reactive protein ; DTNB ; 5 ; 5&prime ; -dithio-bis-2-nitrobenzoic acid ; EDTA ; ethylenediaminetetraacetic acid ; ELISA ; enzyme-linked immunosorbent assay ; HPLC ; high-pressure liquid chromatography ; IFNs ; interferons ; IL ; interleukin ; MS ; multiple sclerosis ; NTPDase ; ectonucleoside triphosphate diphosphohydrolase ; PPMS ; primary progressive form of MS ; RRMS ; rela
• 刊名：Neuroscience
• 出版年：25 April, 2014
• 年：2014
• 卷：266
• 期：Complete
• 页码：266-274
• 全文大小：371 K

文摘

Multiple sclerosis (MS) is one of the main chronic inflammatory diseases of the CNS that cause functional disability in young adults. It has unknown etiology characterized by the infiltration of lymphocytes and macrophages into the brain. The aim of this study was to evaluate the acetylcholinesterase (AChE) activity in lymphocytes and whole blood, as well as butyrylcholinesterase (BChE) and adenosine deaminase (ADA) activities in serum. We also checked the levels of nucleotides, nucleosides, biomarkers of inflammation such as cytokines (interleukin (IL)-1, IL-6, interferon (IFN)-纬, tumor necrosis factor-alpha (TNF-伪) and IL-10) and C-reactive protein (CRP) in serum from 29 patients with the relapsing-remitting form of MS (RRMS) and 29 healthy subjects as the control group. Results showed that AChE in lymphocytes and whole blood as well as BChE, and ADA activities in serum were significantly increased in RRMS patients when compared to the control group (P < 0.05). In addition, we observed a decrease in ATP levels and a significant increase in the levels of ADP, AMP, adenosine and inosine in serum from RRMS patients in relation to the healthy subjects (P < 0.05). Results also demonstrated an increase in the IFN-纬, TNF-伪, IL-1, IL-6 and CRP (P < 0.05) and a significant decrease in the IL-10 (P < 0.0001) in RRMS patients when compared to control. Our results suggest that alterations in the biomarkers of inflammation and hydrolysis of nucleotides and nucleosides may contribute to the understanding of the neurological dysfunction of RRMS patients.