Hydrogen and ethanol fermentation of various carbon sources by immobilized Escherichia coli (XL1-Blue)

详细信息    查看全文

• 作者：Periyasamy Sivagurunathan^a ; Gopalakrishnan Kumar^a ; ^{gopalakrishnanchml@gmail.com" class="auth_mail} ; Chiu-Yue Lin^a ; ^b ; ^{cylin@fcu.edu.tw" class="auth_mail}
• 关键词：Escherichia coli (XL1-Blue) ; Beverage wastewater ; Immobilized cells ; Fermentation ; Soluble metabolic products
• 刊名：International Journal of Hydrogen Energy
• 出版年：24 April, 2014
• 年：2014
• 卷：39
• 期：13
• 页码：6881-6888
• 全文大小：574 K

文摘

In this study, hydrogen and ethanol production by a facultative anaerobic bacterium Escherichia coli XL1-Blue immobilized in calcium-alginate beads have been investigated. Batch fermentations were carried out at mesophilic temperature (35聽掳C) and an initial cultivation pH of 6.5. Firstly, the influence of biomass concentration in terms of dry cell weight (expressed in g DCW/L, range 0.2-1.0) was investigated using fructose (5聽g/L) as a carbon source. The peak hydrogen yield (HY) of 1.17聽mol-H₂/mol-fructose_utilized was obtained at an initial cell concentration of 0.4聽g DCW/L. The hydrogen production potential of other simple carbon sources (glucose and xylose) was evaluated at this optimized cell concentration and peak HY values were attained as 0.96聽mol-H₂/mol-glucose_utilized and 0.69聽mol-H₂/mol-xylose_utilized, respectively. In addition, utilization of the beverage wastewater (BWW) showed the peak cumulative hydrogen production and ethanol concentration of 120聽mL and 5.65聽g/L, attained at the substrate concentration of 20聽g_{(glucose equivalent)}/L. However, peak HY (1.65聽mol-H₂/mol-_{glucose eqivalent utilized}) was observed at low substrate concentration of 5聽g_{(glucose equivalent)}/L. The percentage of sugar utilization of BWW was ranged between 80 and 96.