Schisandra chinensis extract ameliorates nonalcoholic fatty liver via inhibition of endoplasmic reticulum stress

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• 作者：Min-Kyung Jang^a ; ^b ; ^{ckone0506@hanmail.net" class="auth_mail" title="E-mail the corresponding author} ; Jeong Soo Nam^a ; ^b ; ^{hidejsugi@naver.com" class="auth_mail" title="E-mail the corresponding author} ; Ji Ha Kim^a ; ^b ; ^{kjh30433@hanmail.net" class="auth_mail" title="E-mail the corresponding author} ; Ye-Rang Yun^a ; ^b ; ^{yunyerang@pusan.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Chang Woo Han^b ; ^{yeast10@hanmail.net" class="auth_mail" title="E-mail the corresponding author} ; Byung Joo Kim^a ; ^b ; ^{vision@pusan.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Han-Sol Jeong^b ; ^{jhsol33@pusan.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Ki-Tae Ha^b ; ^{hagis@pusan.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Myeong Ho Jung^a ; ^b ; ^{jung0603@pusan.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Schisandra chinensis ; Endoplasmic reticulum stress ; Nonalcoholic fatty liver disease ; Triglyceride accumulation ; Inflammation ; Lipogenesis
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：5 June 2016
• 年：2016
• 卷：185
• 期：Complete
• 页码：96-104
• 全文大小：2355 K

文摘

Schisandra chinensis (Turcz.) Baill. (SC) is a traditional Chinese herbal medicine with diverse pharmacological activities for treatment of various human diseases. Endoplasmic reticulum (ER) stress is associated with the pathogenesis of nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the protective effects of methanol extract of Schisandra chinensis (SC extract) against ER stress-induced NAFLD in vitro and in vivo.

Material and methods

The protective effects of SC extract were examined in tunicamycin- or palmitate-treated HepG2 cells in vitro, and in tunicamycin-injected mice or high fed diet (HFD) obese mice in vivo. Expression of ER stress markers including glucose regulated protein 78 (GRP78), C/EBP homolog protein (CHOP), and X-box-binding protein-1 (XBP-1), and triglyceride accumulation were measured in HepG2 cells and in the liver of mice.

Results

SC extract significantly inhibited expression of tunicamycin-induced ER stress markers in tunicamycin-treated HepG2 cells and in the liver of tunicamycin-injected mice, and it also inhibited tunicamycin-induced triglyceride accumulation. Similar observations were made under physiological ER stress conditions such as in palmitate-treated HepG2 cells and in the liver of HFD obese mice. In addition, SC extract repressed the expression of inflammatory genes and lipogenic genes in palmitate-treated HepG2 cells. Schisandrin, an abundant bioactive lignan in SC extract, inhibited the expression of ER stress markers in tunicamycin-or palmitate-treated HepG2 cells, whereas Gomisin J did not affect ER stress markers.

Conclusions

SC attenuates ER stress and prevents development of NAFLD. SC may be useful as a pharmacological agent for protection against ER stress-induced human diseases.