Embryo transfer manipulation cause gene expression variation in blastocysts that disrupt implantation and offspring rates at birth in rabbit

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• 作者：Maria Desemparats Saenz-de-Juano^a ; Francisco Marco-Jimé ; nez^b ; Jose Salvador Vicente^b ; ^{jvicent@dca.upv.es}
• 关键词：Preimplantation embryo ; Embryo transfer ; OCT4 ; EMP1 ; C1QTNF1 ; TNFAIP6
• 刊名：European Journal of Obstetrics & Gynecology and Reproductive Biology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：207
• 期：Complete
• 页码：50-55
• 全文大小：831 K
• 卷排序：207

文摘

In the current study we aimed to evaluate the effect of embryo transfer on gene expression during pre-implantation development and its consequences on implantation rate, offspring rate at birth and embryonic and fetal losses in the rabbit model.Study designThe mRNA expressions of 8 candidate genes were compared between 6-day-old in vivo-produced embryos (non-manipulated embryos) to those of 6-day-old embryos previously recovery at the third day of development and transferred into recipient rabbit females (manipulated embryos). Furthermore, we compared between both experimental groups the implantation rate and offspring rate at birth and embryonic and fetal losses.ResultsDifferences in transcript abundance of OCT4, C1qTNF1, EMP1 and TNFAIP6 were observed in transferred embryos. In addition, lower implantation and offspring rates at birth were obtained in transferred embryos than in the control group. In addition, embryonic losses were significantly higher in the transferred group than in the control. However, fetal losses were similar between groups.ConclusionThe findings of the current study show that embryo transfer manipulation influenced mRNA expression of late blastocysts prior to implantation, resulting in higher gestational losses as a consequence of faulty embryonic implantation.