Younger patients with COME are more likely to have mucoid middle ear fluid containing mucin MUC5B

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• 作者：Vanessa Duah^a ; Zhen Huang^b ; Stephanie Val^b ; ^c ; Christie DeMason^b ; Marain Poley^b ; ^{marianpoley@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Diego Preciado^b ; ^c ; ^{dpreciad@cnmc.org" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chronic otitis media with effusion ; Mucous ; Serous ; MUC5B ; MUC5AC
• 刊名：International Journal of Pediatric Otorhinolaryngology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：90
• 期：Complete
• 页码：133-137
• 全文大小：385 K

文摘

Chronic otitis media (COM) is one of the most common childhood diseases. Its pathophysiology is complex and multifactorial. The role of specific mucin glycoprotein subtypes in OM is only recently being elucidated.

Objective

To determine the relationship between middle ear fluid mucins and clinical variables of patients needing tympanostomy tubes (TT).

Methods

Middle ear effusions (MEE) from children receiving TT were collected over a 2-year period. Western blot characterization of mucins MUC5B and MUC5AC along with chart review of age, gender, effusion viscosity, hearing loss >30 dB, history of allergies, and/or respiratory disease were performed.

Results

MEE samples from 58 patients were available for analysis. Overall, MUC5B was significantly more often detected in middle ear fluid relative to MUC5AC (90% vs. 51%, p = 0.005). MUC5B presence was statistically associated with mucoid effusions relative to serous effusions (100% vs. 57%, p = 0.0064), MUC5AC presence was not significantly different in mucoid and serous fluid (55.1% vs. 37.5%, p = 0.447). Patients younger than 48 months were more likely to present with mucoid effusion, compared to those older than 48 months of age (p = 0.038). Finally, patients with effusions positive for MUC5B were younger than those with effusions negative for MUC5B (35.1 vs. 76 months, p = 0.045). No other variables correlated to either effusion viscosity or specific mucin content.

Conclusion

Patients younger in age needing TT placement are more likely to present with mucoid effusions, predominantly containing MUC5B mucin. As such, we postulate a distinct pathophysiology for mucoid and serous effusions across ages in children with COM.