In vitro selection of RNA aptamers against CA125 tumor marker in ovarian cancer and its study by optical biosensing

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• 作者：Ilaria Lamberti^a ; Simona Scarano^c ; Carla Lucia Esposito^d ; Antonio Antoccia^a ; ^b ; Giovanni Antonini^a ; ^b ; Caterina Tanzarella^a ; Vittorio De Franciscis^d ; ^{defranci@unina.it" class="auth_mail" title="E-mail the corresponding author} ; Maria Minunni^b ; ^c ; ^{maria.minunni@unifi.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Aptamers ; SELEX ; CA125 ; Surface Plasmon Resonance
• 刊名：Methods
• 出版年：2016
• 出版时间：15 March 2016
• 年：2016
• 卷：97
• 期：Complete
• 页码：58-68
• 全文大小：1660 K

文摘

Early identification of neoplastic diseases is essential to achieve timely therapeutic interventions and significantly reduce the mortality of patients. A well-known biomarker is the Cancer Antigen 125 (CA125) or 16 mucin (MUC 16), a glycoprotein of the human family of mucins, already used for the diagnostic and prognostic evaluation of ovarian cancer. Therefore, the detection of CA125 to now remains a promising tool in the early diagnosis of this tumor. In this paper, we describe the development of RNA aptamers that bind with high affinity the tumor antigen CA125. We performed eight cycles of selection against CA125 purified protein. The selected aptamers were cloned and sequenced and the binding properties of the most promising sequences were studied by Real Time PCR and Surface Plasmon Resonance (SPR) to evaluate their ability in targeting CA125 protein with perspective applications in aptamer-based bioassays.