Gene sequencing and variable site analyzing of coding region of two enterovirus A71 isolates with different clinical phenotypes

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• 作者：Bing-Yu Xie^a ; Chao Xu^a ; Qing Xiong^a ; Meng Zhu^a ; Hao Duan^a ; Yu-Ya Wang^a ; Hao Zhang^a ; Xue-Fei Ni^b ; Ling-Hang Wang^b ; Xing-Wang Li^b ; Tao Shen^a ; Yi-Hong Peng^a ; ^{ypeng78@bjmu.edu.cn}
• 关键词：EV-A71 ; enterovirus A71 ; HFMD ; hand ; foot ; and mouth disease ; CA16 ; Coxsackievirus A16 ; 5&prime ; -UTR ; 5&prime ; -untranslated region ; 3&prime ; -UTR ; 3&prime ; -untranslated region ; RT-PCR ; reverse transcription-polymerase chain reaction ; DMEM ; Dulbecco's modified Eagle's medium ; FBS ; fetal bovine serum ; RD ; rhabdomyosarcoma ; RACE ; rapid amplification of cDNA ends ; CPE ; cytopathologic effect ; ORF ; open reading frame
• 刊名：Infection, Genetics and Evolution
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：45
• 期：Complete
• 页码：83-89
• 全文大小：997 K
• 卷排序：45

文摘

Phylogenetic analysis showed that 19 EV-A71 isolates belonged to sub-genotype C4a. Eight variable amino acid sites were found in two EV-A71 isolates with different clinical manifestations. The potential pathogenicity of EV-A71 seems not to be associated with viral genotype and the amino acid substitution.