Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications
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- 作者：Yang Sia ; b ; Chloé ; Grazona ; Gilles Claviera ; Jutta Riegerc ; Jean-Fré ; dé ; ric Audiberta ; Bianca Sclavib ; sclavi@lbpa.ens-cachan.fr" class="auth_mail" title="E-mail the corresponding author ; Rachel Mé ; allet-Renaulta ; 1 ; rachel.meallet-renault@u-psud.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bacteria ; Fluoresceinamine ; Organic nanoparticles ; Rapid detection ; High-throughput screening (HTS) ; pH detection
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：75
- 期：Complete
- 页码：320-327
- 全文大小：3273 K
文摘
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Two highly water-soluble, biocompatible, stable and bright fluoresceinamine based pH-sensitive nanoparticles (FANPs) are presented.
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These FANPs can be used for a rapid, accurate, reproducible and highly sensitive detection of E. coli bacterial growth.
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These FANPs are very easy to synthesize with a low cost and can be easily used in any laboratory without additional expensive instrumentation or experienced operators.
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These FANPs allow for continuous monitoring for real-time detection of bacterial growth and can be used for screening of a large number of samples for high-throughput applications such as the measurement of the change in bacterial metabolism due to the presence of antibiotics.