An in situ immobilization system for enzymatic cadaverine production was developed.
CadA was immobilized on poly (3-hydroxybutyrate) (P(3HB)) biopolymer granules.
Increased P(3HB) production was observed with phasin-fused CadA.
The thermal stability increased with immobilization.
Repetitive reactions of the CadA–P(3HB) complex were observed.