Melatonin not only restores but also prevents the inhibition of the intestinal Ca^{2 +} absorption caused by glutathione depleting drugs

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• 作者：Vanessa Areco^a ; Valeria Rodriguez^a ; Ana Marchionatti^a ; Agata Carpentieri^a ; ^b ; Nori Tolosa de Talamoni^a ; ^{ntolosatalamoni@yahoo.com.ar" class="auth_mail" title="E-mail the corresponding author} ; ^{ntolosa@biomed.fcm.unc.edu.ar" class="auth_mail" title="E-mail the corresponding author}
• 关键词：O2&minus ;   ; superoxide anion ; BSO ; DL-buthionine-S ; R-sulfoximine ; CB D28k ; calbindin D28k ; CB D9K ; calbindin D9k ; CLDNs ; claudins ; DAB ; 3 ; 3&prime ; -diaminobenzidine ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; GI ; gastrointestinal ; GSH ; glutathione ; GSSG ; oxidized glutathione ; I/R ; Ischemia and reperfusion ; iNOS ; inducible nitric oxide synthase ; MEL ; melatonin ; MEN ; menadione ; NBT ; nitro blue tetrazolium ; NCX1 ; Na+/Ca2  ; + exchanger ; NO ; nitric oxide ; PMCA1b ; plasma membrane Ca2 
• 刊名：Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：197
• 期：Complete
• 页码：16-22
• 全文大小：1161 K

文摘

We have previously demonstrated that melatonin (MEL) blocks the inhibition of the intestinal Ca^2 + absorption caused by menadione (MEN). The purpose of this study were to determine whether MEL not only restores but also prevents the intestinal Ca^2 + absorption inhibited either by MEN or BSO, two drugs that deplete glutathione (GSH) in different ways, and to analyze the mechanisms by which MEN and MEL alter the movement of Ca^2 + across the duodenum. To know this, chicks were divided into four groups: 1) controls, 2) MEN treated, 3) MEL treated, and 4) treated sequentially with MEN and MEL or with MEN and MEL at the same time. In a set of experiments, chicks treated with BSO or sequentially with BSO and MEL or with BSO and MEL at the same time were used. MEL not only restored but also prevented the inhibition of the chick intestinal Ca^2 + absorption produced by either MEN or BSO. MEN altered the protein expression of molecules involved in the transcellular as well as in the paracellular pathway of the intestinal Ca^2 + absorption. MEL restored partially both pathways through normalization of the O₂⁻ levels. The nitrergic system was not altered by any treatment. In conclusion, MEL prevents or restores the inhibition of the intestinal Ca^2 + absorption caused by different GSH depleting drugs. It might become one drug for the treatment of intestinal Ca^2 + absorption under oxidant conditions having the advantage of low or null side effects.