Human white adipocytes express the cold receptor TRPM8 which activation induces UCP1 expression, mitochondrial activation and heat production

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• 作者：Marco Rossato ; Marnie Granzotto ; Veronica Macchi ; Andrea Porzionato ; Lucia Petrelli ; Aless ; ra Calcagno ; Juri Vencato ; Diego De Stefani ; Valentina Silvestrin ; Rosario Rizzuto ; Franco Bassetto ; Raffaele De Caro ; Roberto Vettor
• 关键词：Human adipocyte ; TRPM8 ; UCP1 ; Mitochondria ; Menthol
• 刊名：Molecular and Cellular Endocrinology
• 出版年：5 March, 2014
• 年：2014
• 卷：383
• 期：1-2
• 页码：137-146
• 全文大小：1888 K

文摘

Mammals possess two types of adipose tissue, white (WAT) and brown (BAT). The uncoupling protein 1 (UCP1) is a hallmark of BAT, being the pivotal player for cold-induced thermogenesis. WAT can acquire BAT characteristics with up-regulation of UCP1 after cold exposure or adrenergic stimulation.

In the present study we demonstrated that human white adipocytes express the cold-sensing receptor TRPM8 which activation by menthol and icilin induced a rise in [Ca²⁺]_i and UCP1 expression, increased mitochondrial membrane potential, glucose uptake and heat production. The induction of 鈥渂rown-like鈥?phenotype in human white adipocytes after TRPM8 activation was supported by ultrastructural morphological changes of mitochondrial morphology and of their intracellular localization, with no modifications of the genes regulating mitochondrial biogenesis.

In conclusion human white adipocytes express the cold receptor TRPM8 which activation induces their 鈥渂rowning鈥?supporting a possible role of this receptor in the control of adipose tissue metabolism and body energy balance.