A viral satellite DNA was integrated to a more efficient p1.7A + 2mβ vector.
The TYLCCNV:PDS vector efficiently silenced PDS gene in N. benthamiana plants.
The TYLCCNV:35S vector obviously silenced GFP gene in N. benthamiana (16c) plants.
GFP silencing induced by TYLCCNV:35S was heritable in N. benthamiana (16c) plants.
Highly methylated cytosine of 35S promoter in TYLCCNV:35S-inoculated plants (16c).