Determination of triazine herbicides in seaweeds: Development of a sample preparation method based on Matrix Solid Phase Dispersion and Solid Phase Extraction Clean-up

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• 作者：N. Rodrí ; guez-Gonzá ; lez ; M.J. Gonzá ; lez-Castro ; ^{mjgc@udc.es" class="auth_mail} ; E. Beceiro-Gonzá ; lez ; S. Muniategui-Lorenzo ; D. Prada-Rodrí ; guez
• 关键词：Triazine herbicides ; Seaweeds ; Matrix Solid Phase Dispersion ; Solid Phase Extraction ; High Performance Liquid Chromatography ; Diode Array Detection
• 刊名：Talanta
• 出版年：April, 2014
• 年：2014
• 卷：121
• 期：Complete
• 页码：194-198
• 全文大小：608 K

文摘

A method using dual process columns of Matrix Solid Phase Dispersion (MSPD) and Solid Phase Extraction (SPE) has been developed for extracting and cleaning-up of nine triazine herbicides (ametryn, atrazine, cyanazine, prometryn, propazine, simazine, simetryn, terbuthylazine and terbutryn) in seaweed samples. Under optimized conditions, samples were blended with 2 g of octasilyl-derivatized silica (C₈) and transferred into an SPE cartridge containing ENVI^TM-Carb II/PSA (0.5/0.5 g) as a clean up co-sorbent. Then the dispersed sample was washed with 10 mL of n-hexane and triazines were eluted with 20 mL ethyl acetate and 5 mL acetonitrile. Finally the extract was concentrated to dryness, re-constituted with 1 mL methanol:water (1:1) and injected into the HPLC-DAD system. The linearity of the calibration curves was excellent in matrix matched standards, and yielded the coefficients of determination>0.995 for all the target analytes. The recoveries ranged from 75% to 100% with relative standard deviations lower than 7%. The achieved LOQs (<10 碌g kg^鈭?) for all triazines under study permits to ensure proper determination at the maximum allowed residue levels set in the European Union Legislation. Samples of three seaweeds were subjected to the procedure proving the suitability of MSPD method for the analysis of triazines in different seaweeds samples.