The Structural Basis of Actin Organization by Vinculin and Metavinculin
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- 作者：Laura Y. Kim1 ; Peter M. Thompson2 ; Hyunna T. Lee2 ; Mihir Pershad3 ; Sharon L. Campbell2 ; Gregory M. Alushin1 ; alushing@mail.nih.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：EM ; electron microscopy ; 2D ; two-dimensional ; IHRSR ; iterative helical real-space reconstruction ; FSC ; Fourier shell correlation ; MDFF ; molecular dynamics flexible fitting ; CTF ; contrast transfer function ; 3D ; three-dimensional ; NHLBI ; National Heart ; Lung ; and Blood Institute
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：16 January 2016
- 年：2016
- 卷：428
- 期：1
- 页码：10-25
- 全文大小：2537 K
文摘
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Cryo-electron microscopy reconstructions of vinculin (Vt) and metavinculin (MVt) tail domains bound to F-actin.
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MVt and Vt undergo partial unfolding transitions upon actin engagement.
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This partial unfolding transition is required for actin bundling by Vt.
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Tension will favor the partially unfolded, actin-bound state, reinforcing adhesion through vinculin.