Fabrication of label-free electrochemical impedimetric DNA biosensor for detection of genetically modified soybean by recognizing CaMV 35S promoter
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- 作者：Shanshan Wang ; Qian Liu ; Henan Li ; Yaqi Li ; Nan Hao ; Jing Qian ; Weihua Zhu ; sayman@ujs.edu.cn ; Kun Wang ; wangkun@ujs.edu.cn
- 关键词：Impedimetric DNA biosensor ; Multiwalled carbon nanotube-reduced graphene oxide nanoribbon ; Cauliflower Mosaic Virus 35S ; Genetically modified soybean detection
- 刊名：Journal of Electroanalytical Chemistry
- 出版年：2016
- 出版时间：1 December 2016
- 年：2016
- 卷：782
- 期：Complete
- 页码：19-25
- 全文大小：909 K
- 卷排序：782
文摘
A simple, rapid and label-free biosensor for detection of GM soybean was proposed. The DNA biosensor was constructed without multiple modified or labeled procedures. Wide linear range and low detection limit for CaMV 35S detection was obtained. The proposed method has the merits of good selectivity, reproducibility and stability. The DNA biosensor showed satisfactory results to discriminate GM soybean samples.