Optimization of the production and characterization of lipase from Candida rugosa and Geotrichum candidum in soybean molasses by submerged fermentation
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- 作者：Wilson Galvã ; o de Morais Jú ; niora ; b ; wjunioreq@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Eliana Setsuko Kamimurac ; Eloí ; zio Jú ; lio Ribeiroa ; Benevides Costa Pesselab ; d ; Vicelma Luiz Cardosoa ; Miriam Maria de Resendea
- 关键词：Lipase ; Submerged fermentation ; Candida rugosa ; Geotrichum candidum ; Soybean molasses
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：26-34
- 全文大小：1010 K
文摘
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Production of two different lipases using soybean molasses.
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Central Composite Planning was used to study the effects of the parameters on the lipases production.
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The biochemical characterization and identification of lipases was investigated.
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Enzymes kinetics Km and Vmax was measured from Lineweaver-Burk (double reciprocal).