A cantilever biosensor exploiting electrokinetic capture to detect Escherichia coli in real time

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• 作者：Stephane LeahyAuthor Vitae ; Yongjun Lai ; ^{lai@queensu.ca" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Biosensor ; Cantilever ; Electrokinetic ; Real time ; Escherichia coli ; Piezoelectric
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：238
• 期：Complete
• 页码：292-297
• 全文大小：1576 K

文摘

A cantilever biosensor with integrated electrodes for piezoelectric actuation and electrokinetic capture is used to detect cells in real time. The sandwich electrodes used for electrokinetic capture present advantages over side-by-side electrodes in terms of reducing the gap size between electrodes and the structural mass added to the cantilever. The 5th resonant mode (≈617 kHz) of the biosensor provided a signal-to-noise ratio of 82 within 10 min when E. coli was measured from a stagnant sample with a concentration of 10⁷ cells/ml. The 7th resonant mode (≈1160 kHz) provided a signal-to-noise ratio of 26 within 10 min when E. coli was measured from a flowing sample (400 μl/min) with a concentration of 10⁵ cells/ml. The estimated sensitivity of the 7th resonant mode is 326 fg/Hz based on images of captured cells.