The protective effects of Ribes diacanthum Pall on cisplatin-induced nephrotoxicity in mice

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• 作者：Akhtolkhyn Tilyek^a ; Chengzhi Chai^a ; Xiaoli Hou^a ; ^b ; Baoping Zhou^a ; ^b ; Chunlei Zhang^a ; Zhengyu Cao^a ; ^b ; ^{zycao1999@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Boyang Yu^a ; ^b ; ^{boyangyu59@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ribes diacanthum Pall ; Cisplatin ; Nephrotoxicity/Nephropathology ; Oxidative stress ; Apoptosis
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：3 February 2016
• 年：2016
• 卷：178
• 期：Complete
• 页码：297-306
• 全文大小：8842 K

文摘

Ribes diacanthum Pall. (Saxifragaceae), a Mongolian folk medicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall (RDP) against cisplatin-induced nephrotoxicity.

Methods

The renal injury was modeled by intraperitoneal injection of cisplatin for 5 consecutive days (5 mg/kg). Nephroprotection of RDP was investigated by oral administration of RDP aqueous extract at a daily dose of 40 mg/kg for 14 consecutive days, starting 7 days prior to cisplatin administration.

Results

We demonstrated that pretreatment with RDP aqueous extract protected the mice from death induced by cisplatin administration. RDP treatment also significantly reduced blood urea nitrogen (BUN) and serum creatinine (Cr) levels observed in cisplatin-administrated mice. Histopathological analysis demonstrated that RDP administration protected cisplatin-induced renal tubular cell apoptosis. Further western blotting analysis revealed that RDP significantly reversed cisplatin-increased expression levels of cleaved-Caspase-3, Bax and cisplatin-decreased expression level of Bcl-2 in renal tissue. Finally, RDP markedly enhanced enzyme activities of reduced superoxide dismutase (SOD), Heme oxygenase 1 (HO-1) and catalase (CAT), suppressed lipid peroxidation as well as reactive oxygen species (ROS) production.

Conclusion

We concluded that RDP displayed nephroprotective effects against cisplatin-induced renal tubular cell apoptosis, possibly associated with both enhanced antioxidase activity and suppressed ROS generation. Given the major nephrotoxicity of cisplatin cancer chemotherapy, RDP might be a potential candidate for neoadjuvant chemotherapy.