Exogenous 3,3′-diindolylmethane increases Brassica napus L. seedling shoot growth through modulation of superoxide and hydrogen peroxide content

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• 作者：Arun Gokul^a ; Enrico Roode^a ; Ashwil Klein^b ; Marshall Keyster^a ; ^{mkeyster@uwc.ac.za" class="auth_mail" title="E-mail the corresponding author} ; ^{mkeyster@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ABA ; abscisic acid ; APX ; ascorbate peroxidase ; ANOVA ; analysis of variance ; Cat ; catalase ; DIM ; 3 ; 3&prime ; -diindolylmethane ; GA ; gibberellic acid ; GC ; gas chromatograph ; HPLC ; high-performance liquid chromatography ; HR ; hypersensitive response ; I3C ; indole-3-carbinol ; IAA ; indole-3-acetic acid ; MDA ; malondialdehyde ; NBT ; nitro blue tetrazolium chloride ; ROS ; reactive oxygen species ; SA ; South Africa ; SOD ; superoxide dismutase ; TBARS ; thiobarbituric acid reactive substances
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 June 2016
• 年：2016
• 卷：196-197
• 期：Complete
• 页码：93-98
• 全文大小：854 K

文摘

Brassica napus L. (cv. AV Garnet) seeds were pre-treated with 15 μM 3,3′-diindolylmethane (DIM) to investigate whether DIM could enhance seed germination. Further treatment of seedlings with 15 μM DIM for 14 days explored the effects on seedling shoot growth. Exogenous DIM led to improved germination percentage, increased seedling shoot lengths, and increased fresh and dry weights. Furthermore, DIM triggered induction of superoxide radical (O₂⁻) and hydrogen peroxide (H₂O₂) content however, no change in malondialdehyde (MDA) content and cell death (assessed with Evans Blue assay) was detected for both the control and DIM treated seedling shoots. We also observed increases in superoxide dismutase (SOD) activity and ascorbate peroxidase (APX) activity in response to exogenous DIM, two fundamental enzymes in the control of reactive oxygen species (ROS) in plants. These results indicate that exogenous DIM treatment enhances seed germination and improves seedling shoot growth through possible activation of a reactive oxygen species signalling pathway involving O₂⁻ and H₂O₂ in B. napus.