GA₄ and IAA were involved in the morphogenesis and development of flowers in Agapanthus praecox ssp. orientalis

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• 作者：Di Zhang^a ; Li Ren^a ; Jian-hua Yue^a ; Ling Wang^b ; Li-huan Zhuo^b ; Xiao-hui Shen^a ; ^{shenxh62@sjtu.edu.cn" class="auth_mail}
• 关键词：ABA ; abscisic acid ; An ; anther ; BSA ; bovine serum albumin ; CKs ; cytokinins ; DHZR ; dihydrozeatin riboside ; DHZ9G ; dihydrozeatin 9-glucoside ; DHZMP ; dihydrozeatin-5-monophosphate ; EDAC ; 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimidehydrochloride ; FP ; floral primordia ; GA ; gibberellic acid ; HPLC-ESI-ion trap MS ; high-performance liquid chromatography/electrospray ionization ion trap tandem mass spectrometry ; IAA ; indole-acetic acid ; iP ; N6-isopentenyladenine ; iP7G ; N6-isopentenyladenine-7-glucosid
• 刊名：Journal of Plant Physiology
• 出版年：1 July 2014
• 年：2014
• 卷：171
• 期：11
• 页码：966-976
• 全文大小：4643 K

文摘

The transition from vegetative to reproductive growth represents a major phase change in angiosperms. Hormones play important roles in this process. In this study, gibberellic acid (GA), cytokinins (CKs), indoleacetic acid (IAA), and abscisic acid (ABA) were analyzed during the flowering in Agapanthus praecox ssp. orientalis. Eleven types of endogenous gibberellins in addition to GA₁ were detected in various organs. GA₉ was detected with the highest concentrations, followed by GA₅, GA₈, and GA₁₉. However, GA₄ was the main bioactive GA that was involved in the regulation of flowering. Eight types of endogenous cytokinins were detected in A. praecox ssp. orientalis, and zeatin, zeatin riboside, zeatin-O-glucoside, and N⁶-isopentenyladenosine-5-monophosphate were present at higher levels throughout the study, of which zeatin plays an important role in the development of various organs. IAA increased by 581% in the shoot tips from the vegetative to inflorescence bud stages and had the most significant changes during flowering. Phytohormone immunolocalization analysis suggested that IAA involved in differentiation and development of each floral organs, GA and zeatin play important roles in floret primordia differentiation and ovule development. Using exogenous plant growth regulators proved that GA signaling regulate the scape elongation and stimulate early-flowering, and IAA signaling is involved in the pedicel and corolla elongation and delay flowering slightly.