The Gene Transfer Agent RcGTA Contains Head Spikes Needed for Binding to the Rhodobacter capsulatus Polysaccharide Cell Capsule
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- 作者：Alexander B. Westbye1 ; Kevin Kuchinski1 ; Calvin K. Yip2 ; J. Thomas Beatty1 ; j.beatty@ubc.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：GTA ; gene transfer agent ; SFP ; side fiber protein ; CPS ; capsular polysaccharide ; CBM ; carbohydrate-binding module ; TEM ; transmission electron microscopy
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：428
- 期：2
- 页码：477-491
- 全文大小：1064 K
文摘
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Gene transfer agents are phage-like particles that mediate HGT.
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The RcGTA particles contain head spikes, side fibers and a baseplate-like structure.
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Head spike formation and binding to the bacterial capsule require GhsA and GhsB.
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GhsA and GhsB production is regulated by CckA-ChpT-CtrA and quorum sensing, which also regulate the production of the polysaccharide capsule bound by GhsB-containing RcGTA.
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Capsid-associated proteins on tailed phages that mediate cell recognition may be common.