Enhanced catalytic efficiency of endo-β-agarase I by fusion of carbohydrate-binding modules for agar prehydrolysis
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- 作者：Bassam Alkotainia ; Nam Soo Hanb ; Beom Soo Kima ; bskim@chungbuk.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Endo-β-agarase I ; GH16 ; CBM6 ; CBM13 ; Agar prehydrolysis ; Fusion enzyme
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：93-94
- 期：Complete
- 页码：142-149
- 全文大小：934 K
文摘
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Engineered fusions of catalytic domain of endo-β-agarase I (GH16) and carbohydrate-binding modules (CBM6 and CBM13) were constructed.
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The binding affinity and catalytic efficiency of GH16 were enhanced.
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Fusion GH16-CBM6-CBM13 was used to prehydrolyze melted agar as the first step in saccharification process.