Influence of a family 29 carbohydrate binding module on the activity of galactose oxidase from Fusarium graminearum
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- 作者：Filip Mollerupa ; Kirsti Parikkab ; Thu V. Vuongc ; Maija Tenkanenb ; Emma Mastera ; c ; emma.master@utoronto.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Galactose oxidase ; Carbohydrate binding module ; Galactomannan ; Galactoglucomannan ; Galactoxyloglucan ; Fusion proteins
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：1860
- 期：2
- 页码：354-362
- 全文大小：804 K
文摘
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A family 29 carbohydrate-binding module (CBM) was fused to galactose oxidase (GaO).
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CBM fusion increased GaO binding to galactose-containing polysaccharides.
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C-terminal fusion of CBM29 increased the catalytic efficiency of GaO.
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Varying impacts of CBM29 positioning suggests competing action of native CBM32.