Improved biomass degradation using fungal glucuronoyl—esterases—hydrolysis of natural corn fiber substrate
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- 作者：Clotilde d'Erricoa ; Johan Bö ; rjessonb ; Hanshu Dingb ; Kristian B.R.M. Kroghb ; Nikolaj Spodsbergb ; Robert Madsena ; Rune Nygaard Monradb ; rnmo@novozymes.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：CBM ; carbohydrate binding module ; CE ; carbohydrate esterase ; CV ; column volume ; CuGE ; Cerrena unicolor glucuronoyl esterase ; DDQ ; 2 ; 3-dichloro-5 ; 6-dicyano-1 ; 4-benzoquinone ; DM ; dry matter ; GE ; glucuronoyl esterase ; HL-NREL-PCS ; high-liquor NREL pretreated corn stover ; LCC ; lignin-carbohydrate complex ; NREL-PCS ; NREL pretreated corn stover ; ScGE ; Schizophyllum commune glucuronoyl esterase ; TLC ; thin-layer chromatography ; TrGE ; Trichoderma reesei glucuronoyl esterase ; TrβX ; Trichoderma reesei β-xy
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 February 2016
- 年：2016
- 卷：219
- 期：Complete
- 页码：117-123
- 全文大小：795 K
文摘
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Fungal CE15 GEs were found to improve the enzymatic saccharification of corn fiber.
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GEs degrade ester LCCs between lignin alcohols and glucuronic acids in xylans.
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A preference for bulky arylalkyl substrates mimicking LCCs was observed.
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GEs may act as auxiliary enzymes in enzymatic saccharification of lignocellulosics.
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Hereby GEs improve access of cellulases and hemicellulases to plant poysaccharides.