Toward a suitable structural analysis of gene delivery carrier based on polycationic carbohydrates by electron transfer dissociation tandem mass spectrometry
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- 作者：Cé ; dric Przybylskia ; cedric.przybylski@upmc.fr" class="auth_mail" title="E-mail the corresponding author ; Juan M. Benitob ; Vé ; ronique Bonnetd ; Carmen Ortiz Melletc ; José ; M. Garcí ; a Ferná ; ndezb
- 关键词：Polycationic ; Carbohydrates ; ETD ; Cyclodextrin ; ESI-MS/MS ; Gene delivery
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：948
- 期：Complete
- 页码：62-72
- 全文大小：2671 K
文摘
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The first ETD-MS/MS characterization of polycationic carbohydrate based non-viral gene delivery agents.
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Suitable selection of charge states and fluoranthene reagent time improves ETD fragmentation efficiency.
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ETD with SA can complete structural deciphering of some building blocks which is not possible with CID only.
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ETD based fragmentation is more efficient with long grafted polycationic arms.
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MS/MS results can be used to correlate nitrogen/phosphorus ratio (N/P) in DNA compaction.