Pharmacological characterisation of pyrimidinoceptor responses in NG108-15 cells

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• 作者：Sak ; Katrin ; Samuel ; Kü ; lli ; Kelve ; Merike ; Webb ; Tania E.
• 关键词：NG108-15 cell line ; Pyrimidinoceptor ; UTP-preferring receptor ; Inositol phospholipid ; Adenosine
• 刊名：European Journal of Pharmacology
• 出版年：2001
• 出版时间：March 16, 2001
• 年：2001
• 卷：415
• 期：2-3
• 页码：127-133
• 全文大小：259 K

文摘

In the present study, the P2Y receptor(s) mediating the effects of the pyrimidines UTP and UDP on phospholipase C activation in the mouse neuroblastoma×rat glioma hybrid cell line NG108-15 was investigated. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) analysis detected transcripts for the P2Y₆ and P2Y₂ receptors, but not for P2Y₁ and P2Y_4. UTP and UDP were equipotent agonists and their effects were partially additive. Suramin, reactive blue 2 and pyridoxal phosphate-6-azophenyl-2′,4′disulfonic acid (PPADS) antagonised the phospholipase C response to both UTP and UDP. High micromolar concentrations of adenosine, 2-p-(2-carboxyethyl)phenethylamino-5′-N-ethylcarboxamidoadenosine (CGS-21680), 2′,3′-O-isopropylideneadenosine (iPAdo) and adenosine 3′:5′-cyclic monophosphate (3′,5′-cAMP) were able to antagonise the effect of UTP on phospholipase C but not that of UDP. The additivity of the UTP and UDP responses, novel P2 receptor antagonist profile and the distinguishing action of adenosine may indicate the expression of a pyrimidine selective P2Y receptor in addition to the P2Y₆ type in these cells.