Sub-lethal concentrations of CdCl2 disrupt cell migration and cytoskeletal proteins in cultured mouse TM4 Sertoli cells
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- 作者：Biola F. Egbowon ; biola.egbowon@ntu.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Wayne Harris ; Gordon Arnott ; Chris Lloyd Mills ; Alan J. Hargreaves ; alan.hargreaves@ntu.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：AIDA ; Advanced Image Data Analyser ; BSA ; bovine serum albumin ; CdCl2 ; cadmium chloride ; CFSE ; carboxyfluorescein diacetate succinimidyl ester ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; DMSO ; dimethyl sulfoxide ; FITC ; fluorescein isothiocyanate ; H and E ; haematoxylin and eosin ; IC50 ; inhibiting concentration (50%) ; LDH ; lactate dehydrogenase ; MFs ; microfilaments ; MTs ; microtubules ; MTT ; 3-[4 ; 5-dimethylthiazol-2-yl]-2 ; 5-diphenyltetrazolium bromide ; PBS ; phosphate buffered saline ; SDS ; sodium dode
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：32
- 期：Complete
- 页码：154-165
- 全文大小：1709 K
文摘
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CdCl2 reduced cultured mouse TM4 Sertoli cell viability dose and time dependently.
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A sublethal level of 1 μM CdCl2 disrupted cell migration and the cytoskeleton.
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Similar effects were exerted by 12 μM CdCl2 but were associated with cell death.
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Such levels of Cd2 + have been detected by others in human and rodent tissues.
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At these levels Cd2 + may disrupt Sertoli cell migration and viability in vivo.