Genetic variation of C18:1 and C18:2 isomers in sheep milk fat

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• 作者：Bianca Moioli^a ; ^{bianca.moioli@entecra.it} ; Giovanna Contarini^b ; Lorraine Pariset^c ; Cinzia Marchitelli^a ; Alessandra Crisà ; ^a ; Gennaro Catillo^a ; Francesco Napolitano^a
• 关键词：CLA ; conjugated linoleic acid ; DGAT1 ; Type 1 acyl CoA ; diacylglycerol acyltransferase ; DGAT2 ; type 2 acyl CoA ; diacylglycerol acyltransferase ; FA ; fatty acid ; FAME ; fatty acid methyl ester ; FASN ; fatty acid synthetase ; GHR ; growth hormone receptor ; IGF1 ; in
• 刊名：Small Ruminant Research
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：103
• 期：2-3
• 页码：187-193
• 全文大小：267 K

文摘

The aim of the present study was to evaluate the genetic variation of milk fat CLA, together with the variation of CLA precursors, including the individual PUFA that are potentially involved in the metabolic pathway of CLA. The allelic substitution effect, upon these FA, was estimated for a number of polymorphisms, that are located within 9 candidate genes known to influence milk FA partitioning. Sheep milk was chosen because it contains higher total fat percentage, and higher CLA levels per gram fat, than milk of other ruminants. Milk FAME analyses were performed on individual milk samples of sheep from three different breeds. Our results reflect the association of three genes (alpha-1-antichymotrypsin; diacylglycerol O-acyltransferase homolog-2; and zona pellucida glycoprotein-2) with the values of both linolenic FA and CLA; of three more genes (insulin-like growth factor I; lecithin-cholesterol acyltransferase and propionyl coenzyme A carboxylase, beta polypeptide) with the variability of linolenic FA; and of one gene, the fatty acid synthetase with the variability of CLA and stearic FA. The current study might contribute to the understanding of the metabolic pathway of PUFA in sheep milk, providing suggestions to modify milk fat composition through selection.