Sequential on-line C-terminal sequencing of peptides based on carboxypeptidase Y digestion and optically gated capillary electrophoresis with laser-induced fluorescence detection
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- 作者：Miaomiao Tian1 ; Ning Zhang1 ; Xiaoxia Liu ; Liping Guo ; Li Yang ; yangl330@nenu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：C-terminal sequencing ; Peptide ; Carboxypeptidase Y ; Capillary electrophoresis
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：12 August 2016
- 年：2016
- 卷：1459
- 期：Complete
- 页码：152-159
- 全文大小：2070 K
文摘
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A novel method for on-line C-terminal sequencing of peptides has been developed.
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Released AAs are sequentially monitored during the entire progress of CPY digestion.
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Sequence of a peptide can be determined with just one OGCE run.
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The method exhibits high repeatability and efficiency for peptide sequencing.