Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line

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• 作者：Jeeranan Manokawinchoke ; Thanaphum Osathanon ; ^{thanaphum.o@chula.ac.th" class="auth_mail" title="E-mail the corresponding author} ; Prasit Pavasant ; ^{prasit215@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Notch signaling ; Oral squamous cell carcinoma ; Osteoprotegerin ; DAPT ; Jagged1
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：6
• 期：8
• 页码：692-697
• 全文大小：618 K

文摘

To investigate the influence of Notch signaling on osteoprotegerin (OPG) expression in a human oral squamous cell carcinoma cell line.

Methods

Activation of Notch signaling was performed by seeding cells on Jagged1 immobilized surfaces. In other experiments, a γ-secretase inhibitor was added to the culture medium to inhibit intracellular Notch signaling. OPG mRNA and protein were determined by real-time PCR and ELISA, respectively. Finally, publicly available microarray database analysis was performed using connection up- or down-regulation expression analysis of microarrays software.

Results

Jagged1-treatment of HSC-4 cells enhanced HES1 and HEY1 mRNA expression, confirming the intracellular activation of Notch signaling. OPG mRNA and protein levels were significantly suppressed upon Jagged1 treatment. Correspondingly, HSC-4 cells treated with a γ-secretase inhibitor resulted in a significant reduction of HES1 and HEY1 mRNA levels, and a marked increase in OPG protein expression was observed. These results implied that Notch signaling regulated OPG expression in HSC-4 cells. However, Jagged1 did not alter OPG expression in another human oral squamous cell carcinoma cell line (HSC-5) or a human head and neck squamous cell carcinoma cell line (HN22).

Conclusions

Notch signaling regulated OPG expression in an HSC-4 cell line and this mechanism could be cell line specific.