Effects of human Dachshund homolog 1 on the proliferation, migration, and adhesion of squamous cell carcinoma of the tongue

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• 作者：Li Zhang ; MD^a ; Cheng-Qin Wang ; MD ; PhD^a ; ^b ; ^{Wangcq1121@126.com" class="auth_mail" title="E-mail the corresponding author} ; Fen Liu ; MD^a ; Zuo-Qing Dong ; MD ; PhD^c ; Peng Zhao ; MD^b ; Xian-ning Dong ; MD^b ; Fengcai Wei ; MD^c ; Xun Qu ; MD ; PhD^d ; Feng-Gang Xiang ; MD^a ; ^b ; ^{Xiangfenggang@163.com" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：121
• 期：1
• 页码：58-66
• 全文大小：2483 K

文摘

To investigate the expression and role of human Dachshund homolog 1 (DACH1) in the tongue squamous cell carcinoma (TSCC).

Study Design

To explore the expression, regulation, and mechanism of DACH1 in TSCC, nine samples of fresh tumor and adjacent tissues, 51 samples of paraffin-embedded TSCC and paired adjacent tissues, and TSCC cell line SCC-25 were examined. Immunohistochemistry, real-time polymerase chain reaction, Western blot, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, Transwell, adhesion assays, and flow cytometry were used.

Results

The DACH1 expression level was significantly lower in tumors than in the adjacent tissues, and such low expression was associated with poor differentiation of tumors, late clinical stage, and lymph node metastasis. Moreover, overexpression of DACH1 might promote apoptosis and inhibit the proliferation, migration, and adhesion of SCC-25 cells.

Conclusions

DACH1 may be a potential molecular target for the therapy of recurrent and metastatic TSCC.