Dynamic transcription of ubiquitin genes under basal and stressful conditions and new insights into the multiple UBC transcript variants
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- 作者：Marzia Bianchi ; marzia.bianchi@uniurb.it" class="auth_mail" title="E-mail the corresponding author ; Elisa Giacomini ; Rita Crinelli ; Lucia Radici ; Elisa Carloni ; Mauro Magnani
- 关键词：Act D ; actinomycin D ; ALLN ; n-acetyl-l-leucyl-l-leucyl-l-norleucinal ; ATCC ; American Type Culture Collection ; B2M ; beta2-microglobulin ; bp ; base pair(s) ; cDNA ; DNA complementary to RNA ; Ct ; cycle threshold ; DMSO ; dimethylsulfoxide ; DUB ; deubiquitinating enzyme ; E1 ; ubiquitin-activating enzyme ; E2 ; ubiquitin-conjugating Enzyme ; E3 ; ubiquitin ligase ; ELISA ; enzyme-linked immunosorbent assay ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; GUSB ; beta glucuronidase ; HPRT1 ; hypoxanthine phosphoribos
- 刊名：Gene
- 出版年：2015
- 出版时间：15 November 2015
- 年：2015
- 卷：573
- 期：1
- 页码：100-109
- 全文大小：821 K
文摘
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Polyubiquitin genes UBC and UBB mainly contribute to Ub content in basal conditions.
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UBC is the most responsive gene to differently induced proteotoxic stress.
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Ub ribosomal fusion genes are not involved in the proteotoxic stress response.
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UV-induced genotoxic stress acts post-transcriptionally by stabilizing Ub mRNAs.
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Multiple transcription start sites were identified within the UBC promoter.