Oxidative damage in trout erythrocyte in response to “in vitro” copper exposure

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• 作者：Donatella Fedeli ; Manuel Carloni ; Giancarlo Falcioni
• 关键词：Copper ; Trout erythrocyte ; Hemoglobin ; Hemolysis ; DNA damage
• 刊名：Marine Environmental Research
• 出版年：2010
• 出版时间：April 2010
• 年：2010
• 卷：69
• 期：3
• 页码：172-177
• 全文大小：271 K

文摘

The oxidative action of copper on different trout Oncorhynchus mykiss erythrocyte components was studied. The results indicate that:

– cupric ions differently influence the oxidative status of two trout hemoglobin components I and IV (HbI and HbIV) having different structural and functional properties;

– reactive oxygen species (ROS) production associated with hemoglobins autoxidation is not influenced by the presence of copper sulfate;

– the susceptibility to hemolysis increases in the presence of copper only when the erythrocyte suspension is incubated in air; the effect of copper is almost absent for carbon monoxide-saturated erythrocyte suspensions;

– DNA damage due to copper was not observed in our experimental conditions.

The data obtained are important for the analysis of the environmental risks produced by copper on fish.