- 作者：Raquel Abad ; Clara I. Agudelo ; M. Cristina Br ; ileone ; Grettel Chanto ; Jean Marc Gabastou ; Juan Carlos Hormazabal ; M. Cecilia O Gorla ; Aurora Maldonado ; Jaime Moreno ; Erwan Muros-Le Rouzic ; Robert Lersch ; Mabel Regueira ; Celia Salcedo ; Cecilia Sorhouet ; Julio A. Vá ; zquez
- 关键词：Meningococci ; Serogroup Y ; Molecular characterization
- 刊名：Journal of Infection
- 出版年：2009
- 出版时间：August 2009
- 年：2009
- 卷：59
- 期：2
- 页码：104-114
- 全文大小：325 K
Summary
ObjectivesTo improve the understanding of serogroup Y invasive meningococcal disease (IMD) in Latin America, particularly IMD molecular epidemiology; 166 Y serogroup isolates received at the National Reference Laboratories of Argentina, Brazil, Chile, Colombia, and Costa Rica during 2000–2006 were characterized by their molecular markers.Methods
This analysis included serological assays to determine serogroup/serotype/serosubtype, DNA sequencing and genotyping of the porB and/or porA genes, multilocus sequence typing (MLST) and fetA allele determination.
Results
Sixteen different antigenic combinations were observed. Sixty-two (37.3 % ) isolates were NT:P1.5 and 36 (21.7 % ) isolates were 14:NST. Thirty-two different STs appeared, but 3 STs (ST-1624, ST-23, and ST-5770) accounted for 69.9 % (116) of the strains.
Most of the IMD isolates belonged to the ST-23, ST-167 clonal complexes or the group composed by ST-5770 and related STs.
Conclusions
Isolates obtained in Colombia and Costa Rica were similar to that of the United States, in that most sequence types belonged to the ST-23 clonal complex. IMD isolates found in Argentina appear to be the result of an independent event and did not spread from nearby countries, being the sequence type ST-1624 (ST-167 clonal complex) the most frequently found. We were unable to correlate an antigenic shift of outer membrane proteins with an increase of serogroup Y meningococcal cases in our collection of isolates.